Automatic Fluid Injection Sampler AFIS


Novel In Situ Water Sampling And Fluid Injection System



Microorganisms are the driving catalysts of virtually all biogeochemical cycles on this planet. Therefore, knowledge regarding microbial abundances, diversities and activities in the environment is of fundamental interest not only to microbiologists, but also for understanding globally important element cycles. One cultivation-independent approach to deduce those prokaryotic metabolic functions is to analyse metatranscriptomes.

The Automatic Fluid Injection Sampler AFIS provides a novel technique to enhance sampling technology for studying microbially driven biogeochemical processes in the environment by the analysis of metatranscriptomes (messenger RNA).

Transcripts degrade fast – within seconds to minutes. It is known that their abundance patterns detected in nature are subject to considerable modification not only due to environmental changes but simply as a result of sampling procedures. Traditional sampling from a ship using conventional water sampling bottles usually takes many minutes to some hours, providing plenty of time for modification.

To overcome this problem, AFIS takes samples and rapidly fixes water directly in the original environment and thereby instantaneously conserves the gene expression profile in situ. This allows a reliable evaluation of microbially driven processes based on metatranscriptomics.

Additional applications are expected in the field of conventional water sampling where immediate fixation of biological matters or chemical substances inside the sample is needed.

The single-bottle version AFISsingle has been designed to be operated inside common rosette water sampling systems or as stand-alone instrument when mounted to hydrographic wires or other subaqueous structures like landers, ROVS etc.

The AFIS results from a cooperation with Leibniz-Institute for Baltic Sea Research Warnemuende IOW.


  • High-pressure injection for fast fixation of the sample within seconds
  • Optimised injection system for even distribution of injection fluid
  • User-selectable injection fluid
  • Pressure-less handling of injection fluid
  • Programmable volume of injection fluid
  • Programmable schedule for sampling procedure
  • For use inside common rosette water sampling systems
Spare Parts

Injection fluid bags


for AFISsingle

material: 5-layer thermoplastic polyolefin
(free from PVC) Set of 10 pcs.

Technical Data 
Dimensions20 x 20 x 65 cm
Empty weight20 kg
Max. operational water depthstandard 3000 m,
optional 6000 m
Materialtechnical ceramics,
titanium, stainless steel AISI 316,
Power supplylithium iron phosphate (LiFePO4),
2500 mAh
Protocol memoryup to 100 operation protocols with
complete nominal and actual sampling scenario
Sampling scenariomechanically activated (by rosette),
depth-, time- or motion-dependent (programmable)
Sampler volume2000 ml
Volume of injection fluidup to 250 ml (programmable)
Injection pressure700 ... 500 kPa (100 ... 70 psi)
Ordering Information
436 430AFISsingle
Automatic Fluid Injection Sampler
Single-bottle version with 2 l capacity
integrated pressure sensor,
power supply: rechargeable lithium iron
phosphate batteries (LiFePO4), 2500 mAh
Windows based OceanLab3 software included
436 430-001Injection fluid bags for AFISsingle,
material: 5-layer thermoplastic polyolefin
(free from PVC) Set of 10 pcs.